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Part:BBa_K088005:Design

Designed by: Hai Chen   Group: iGEM08_CPU-NanJing   (2008-10-13)


pL+lacI+cI+GFP+Pr+mRFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal AgeI site found at 2523
    Illegal AgeI site found at 2635
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 1672


Design Notes

In the absent of lactose or IPTG,mRFP is expressed. In the presence of lactose or IPTG,GFP is expressed, while mRFP not.



Source

R0010+J24679+C0051+E0240+I763007

References